Anticorps Polyclonal de lapin anti-HEXIM1

• Phare
• Validé par KD/KO

HEXIM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 15676-1-AP

Synonymes

Cardiac lineage protein 1, CLP1, EDG1, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1

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Galerie de données de validation

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  WB analysis using 15676-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15676-1-AP (HEXIM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15676-1-AP

  WB result of HEXIM1 antibody (15676-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEXIM1 transfected HeLa cells.

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  IP experiment of HeLa using 15676-1-AP

  IP result of anti-HEXIM1(IP:15676-1-AP, 4ug; Detection:15676-1-AP 1:5000) with HeLa cells lysate 1880 ug.

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  IHC staining of human breast cancer using 15676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15676-1-AP (HEXIM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15676-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15676-1-AP (HEXIM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15676-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEXIM1 antibody (15676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 15676-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human HEXIM1 (15676-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15676-1-AP cible HEXIM1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HEXIM1 Protéine recombinante Ag8144
• Nom complet: hexamethylene bis-acetamide inducible 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC006460
• Symbole du gène: HEXIM1
• Identification du gène (NCBI): 10614
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 65-70 kDa and 54 kDa ( PMID: 33627647, PMID: 20976203, PMID: 28254838).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	IF	Adv Sci (Weinh); cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.; Authors - Zeyun Mi
mouse	WB,IP	Circ Res; Positive transcription elongation factor b activity in compensatory myocardial hypertrophy is regulated by cardiac lineage protein-1.; Authors - Espinoza-Derout Jorge J
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Nucleic Acids Res; HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.; Authors - Sebastian Eilebrecht
human	WB	MBio; Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.; Authors - Juanita Von Dwingelo

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