Anticorps Polyclonal de lapin anti-HIF-1 alpha

• Phare
• Validé par KD/KO

HIF-1 alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

510

Avis (5)

N° de cat : 20960-1-AP

Synonymes

ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF-1, HIF1 ALPHA, HIF-1 alpha, HIF1A, HIF-1α, Member of PAS protein 1, MOP1, PASD8

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Galerie de données de validation

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  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20960-1-AP

  WB result of HIF-1 alpha antibody (20960-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIF-1 alpha transfected HeLa cells. Sample 1: non-treated sh-Control transfected HeLa cells, Sample 2: Cobalt Chloride treated sh-Control transfected HeLa cells, Sample 3: Cobalt Chloride treated sh-HIF-1 alpha transfected HeLa cells.

• 

  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20960-1-AP

  Untreated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 20960-1-AP (HIF1a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20960-1-AP

  IP Result of anti-HIF1a (IP:20960-1-AP, 4ug; Detection:20960-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF1a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20960-1-AP (HIF1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20960-1-AP (HIF1A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20960-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20960-1-AP (HIF1a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 20960-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20960-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using HIF1a antibody (20960-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 20960-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using HIF-1 alpha antibody (20960-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 20960-1-AP

  1X10^6 HeLa cells were stained with 0.2ug HIF1a antibody (20960-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées au chlorure de cobalt, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer de la thyroïde humain, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa traitées au chlorure de cobalt, cellules HepG2 traitées au chlorure de cobalt
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 44 publications below
• WB: See 426 publications below
• IHC: See 98 publications below
• IF: See 68 publications below
• IP: See 11 publications below
• CoIP: See 10 publications below
• ChIP: See 13 publications below

Informations sur le produit

20960-1-AP cible HIF-1 alpha dans les applications de WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain, porc, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HIF-1 alpha Protéine recombinante Ag15198
• Nom complet: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
• Masse moléculaire calculée: 826 aa, 93 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC012527
• Symbole du gène: HIF1A
• Identification du gène (NCBI): 3091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .

Publications

Species	Application	Title
	WB	Gastroenterology; Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells; Authors - Decai Wang
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li
	IF	Cancer Cell; Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity; Authors - Agnieszka Chryplewicz
		Nat Cell Biol; A single-cell transcriptomic landscape of the lungs of patients with COVID-19.; Authors - Si Wang
	WB	ACS Nano; Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling; Authors - Bide Tong
human		Mol Cell; KDM3A Senses Oxygen Availability to Regulate PGC-1α-Mediated Mitochondrial Biogenesis.; Authors - Xu Qian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carlo (Verified Customer) (01-18-2023)

The quality of the band in western blot is well defined

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Neuroblastoma cell line

FH

Davide (Verified Customer) (09-20-2022)

The image uploaded refers to a hypoxic condition O2<1%. The antibody signal was aspecific and generated aspecific binding in the middle of the organoid.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Cholangiocyte organoids

HIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP)

FH

Christian (Verified Customer) (01-07-2022)

Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes).

• Applications: Western Blot
• Cell Tissue Type: human chondrocytes

HIF-1 alpha Antibody Western Blot validation ( dilution) in human chondrocytes (Cat no:20960-1-AP)

FH

Diane (Verified Customer) (02-17-2021)

Separated on a 7.5% SDS PAGE gel, transferred to nitrocellulose membrane. Block with 5% dry milk/PBST at room temperature for one hour. 1:1000 dilution of the primary antibody in blocking solution. Incubated overnight at 4 degrees C on a rocker platform. Secondary antibody 1:1000 secondary (anti rabbit HRP) incubation for two hours at room temperature. Visualization with the Opti-4CN chromogenic substrate kit (BioRad).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: primary glioblastoma cell lysates

HIF-1 alpha Antibody Western Blot validation (1:1000 dilution) in primary glioblastoma cell lysates (Cat no:20960-1-AP)

FH

Chun (Verified Customer) (12-05-2019)

A good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells

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