Anticorps Polyclonal de lapin anti-HINFP

• Phare
• Validé par KD/KO

HINFP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 10066-2-AP

Synonymes

MIZF, Methyl-CpG-binding protein 2-interacting zinc finger protein, MBD2-interacting zinc finger protein, Histone nuclear factor P, HiNF-P

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Galerie de données de validation

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  WB analysis using 10066-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10066-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10066-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 10066-2-AP

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

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  IP experiment of L02 using 10066-2-AP

  IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug.

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  IF Staining of HeLa using 10066-2-AP

  Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of L02 using 10066-2-AP

  Immunofluorescent analysis of L02 cells using 10066-2-AP (HINFP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IF/ICC: cellules HeLa, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10066-2-AP cible HINFP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HINFP Protéine recombinante Ag0115
• Nom complet: histone H4 transcription factor
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC001073
• Symbole du gène: HINFP
• Identification du gène (NCBI): 25988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

Publications

Species	Application	Title
human	WB,IHC,IF	Oncogene; Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.; Authors - Xianchong Zheng ; KO Validated

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