Anticorps Monoclonal anti-HIP1

• Phare
• Validé par KD/KO

HIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

3D3G3

N° de cat : 67163-1-Ig

Synonymes

HIP 1, HIP I, HIP1, ILWEQ

---

Galerie de données de validation

• 

  WB analysis using 67163-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67163-1-Ig

  WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells.

• 

  WB analysis of mouse brain using 67163-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67163-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67163-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 67163-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules PC-3, tissu cérébral de porc, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67163-1-Ig cible HIP1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HIP1 Protéine recombinante Ag17982
• Nom complet: huntingtin interacting protein 1
• Masse moléculaire calculée: 1037 aa, 116 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC110545
• Symbole du gène: HIP1
• Identification du gène (NCBI): 3092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

{{ptg:RelatedPrimaryAntibodies}}