Anticorps Polyclonal de lapin anti-Histone H1

Histone H1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 18201-1-AP

Synonymes

HIST1H1A, Histone, HIST1, H1F1, H1-1

---

Galerie de données de validation

• 

  WB analysis of mouse thymus using 18201-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (HIST1H1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18201-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18201-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18201-1-AP (Histone H1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 18201-1-AP

  IP result of anti-Histone H1 (IP:18201-1-AP, 4ug; Detection:18201-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

• 

  IHC staining of human stomach using 18201-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18201-1-AP (Histone H1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu de thymus de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

18201-1-AP cible Histone H1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H1 Protéine recombinante Ag12704
• Nom complet: histone cluster 1, H1a
• Masse moléculaire calculée: 215 aa, 22 kDa
• Poids moléculaire observé: 28 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC101593
• Symbole du gène: Histone H1
• Identification du gène (NCBI): 3024
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Cell Calcium; IN BRAIN POST-ISCHEMIC PLASTICITY, Na+/Ca2+ EXCHANGER 1 AND Ascl1 INTERVENE IN MICROGLIA-DEPENDENT CONVERSION OF ASTROCYTES INTO NEURONAL LINEAGE.; Authors - Antonella Casamassa
mouse	WB,IP	Int J Mol Sci; The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions; Authors - Ralf Kleene
mouse	IF	Int J Mol Sci; The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions; Authors - Gabriele Loers
	WB	Pharmacol Res; YAP K236 acetylation facilitates its nucleic export and deprived the protection against cardiac hypertrophy in mice; Authors - Panxia Wang

{{ptg:RelatedPrimaryAntibodies}}