Anticorps Polyclonal de lapin anti-Histone H2B

Histone H2B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 15857-1-AP

Synonymes

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

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Galerie de données de validation

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  WB analysis using 15857-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15857-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15857-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15857-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15857-1-AP

  IP Result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 15857-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining using 15857-1-AP

  Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules U2OS, tissu pancréatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: N/A,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 3 publications below

Informations sur le produit

15857-1-AP cible Histone H2B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Histone H2B Protéine recombinante Ag7811
• Nom complet: histone cluster 2, H2be
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC005827
• Symbole du gène: HIST2H2BE
• Identification du gène (NCBI): 8349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Nat Nanotechnol; Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.; Authors - Tanmoy Saha
human	IF	Nat Biomed Eng; Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues; Authors - Su-Jin Heo
human	WB	Oncogene; HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.; Authors - Laura Monteonofrio
human	WB	Elife; Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.; Authors - William F Richter
human	WB	Mol Cell Proteomics; Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.; Authors - Rui Sun
human	WB	Cell Discov; USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.; Authors - Jialun Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gunjita (Verified Customer) (01-06-2020)

N/A

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

FH

Aurelie (Verified Customer) (11-19-2018)

strong signal around 20-25kda in mouse cortex (25µg) at 1:1000, (1:2000 should be ok as well)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: mouse cortex