Anticorps Monoclonal anti-HLA class I ABC

• Phare
• Validé par KD/KO

HLA class I ABC Monoclonal Antibody for WB, IHC, IF/ICC, FC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, FC, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

CloneNo.

5C5B7

N° de cat : 66013-1-Ig

Synonymes

HLA-A, HLA class I histocompatibility antigen, A alpha chain, HLA class I (HLA-A), HLA class I, HLA A

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Galerie de données de validation

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  WB analysis using 66013-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of U-937 using 66013-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66013-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66013-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66013-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66013-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human white blood using 66013-1-Ig

  human white blood cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 66013-1-Ig

  pig spleen tissue was subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66013-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66013-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66013-1-Ig (HLA class I ABC antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 66013-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA class I ABC antibody (66013-1-Ig, Clone: 5C5B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Raji using 66013-1-Ig

  1X10^6 Raji cells were surface stained with 0.4 ug Anti-Human HLA class I ABC (66013-1-Ig, Clone:5C5B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules NCCIT, cellules Raji, cellules U-937, globules blancs humains, tissu splénique de porc
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Raji,
• Résultats positifs en cytométrie: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

66013-1-Ig cible HLA class I ABC dans les applications de WB, IHC, IF/ICC, FC, IP, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HLA class I ABC Protéine recombinante Ag7488
• Nom complet: major histocompatibility complex, class I, A
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: BC003069
• Symbole du gène: HLA-A
• Identification du gène (NCBI): 3105
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	IF	Front Med; Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis; Authors - Weiwei Xian
		Oncotarget; Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells.; Authors - Jianlong Li
human	WB	J Tissue Eng Regen Med; Sequential hydrophile and lipophile solubilization as an efficient method for decellularization of porcine aortic valve leaflets: structure, mechanical property and biocompatibility study.; Authors - Wei-Hua Qiao
human	WB	J Biol Chem; The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.; Authors - Qi Wen Deng ; KD Validated
pig	IHC	Tissue Eng Part A; DECELLULARIZATION OF MASSIVE BONE ALLOGRAFTS BY PERFUSION: A NEW PROTOCOL FOR TISSUE ENGINEERING; Authors - Robin Evrard
human	IHC	Asian Pac J Cancer Prev; Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China.; Authors - Zhao Xiaogang X

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