Anticorps Polyclonal de lapin anti-HLA class I (HLA-C)

HLA class I (HLA-C) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15777-1-AP

Synonymes

HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw

---

Galerie de données de validation

• 

  WB analysis using 15777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 15777-1-AP

  IP result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 15777-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15777-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HLA class I (HLA-C) antibody (15777-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15777-1-AP cible HLA class I (HLA-C) dans les applications de WB, IHC, IF/ICC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA class I (HLA-C) Protéine recombinante Ag8461
• Nom complet: major histocompatibility complex, class I, C
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC007814
• Symbole du gène: HLA class I (HLA-C)
• Identification du gène (NCBI): 3107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Publications

Species	Application	Title
human	WB	J Matern Fetal Neonatal Med; HLA-C promotes proliferation and cell cycle progression in trophoblast cells; Authors - Hong Lv
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang
human	Cell treatment	Nature; Shifts in receptors during submergence of an encephalitic arbovirus; Authors - Wanyu Li
mouse	IF	Neuroscience; HLA is a potent immunoinflammatory target in asymptomatic Alzheimer's disease; Authors - Yingwei Zheng
mouse	WB	Nat Commun; Neoantigen enriched biomimetic nanovaccine for personalized cancer immunotherapy; Authors - Yuwei Li

{{ptg:RelatedPrimaryAntibodies}}