Anticorps Polyclonal de lapin anti-HLA-DRA

HLA-DRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 17221-1-AP

Synonymes

MHC class II antigen DR, HLA-DRA1, HLA-DR, HLA DRB, HLA DRA1

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Galerie de données de validation

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  WB analysis using 17221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17221-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17221-1-AP (HLA-DRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 17221-1-AP

  IP result of anti-HLA-DRA (IP:17221-1-AP, 4ug; Detection:17221-1-AP 1:1000) with Raji cells lysate 3200 ug.

• 

  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17221-1-AP (HLA-DRA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 17221-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 17221-1-AP (HLA-DRA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 17221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRA antibody (17221-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos, tissu splénique de rat
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IHC: tissu d'appendicite humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Informations sur le produit

17221-1-AP cible HLA-DRA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-DRA Protéine recombinante Ag11044
• Nom complet: major histocompatibility complex, class II, DR alpha
• Masse moléculaire calculée: 254 aa, 29 kDa
• Poids moléculaire observé: 32-34 kDa
• Numéro d’acquisition GenBank: BC032350
• Symbole du gène: HLA-DRA
• Identification du gène (NCBI): 3122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
rat	IF	Aging Cell; Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.; Authors - Zhi-Gang Wang
human	WB	Front Immunol; An Immunological Perspective: What Happened to Pregnant Women After Recovering From COVID-19?; Authors - Sijia Zhao
human	IF	Front Immunol; Functional Change of Effector Tumor-Infiltrating CCR5+CD38+HLA-DR+CD8+ T Cells in Glioma Microenvironment.; Authors - Pin-Yuan Chen
rat	IHC	Mol Med; Correction of immunosuppression in aged septic rats by human ghrelin and growth hormone through the vagus nerve-dependent inhibition of TGF-β production.; Authors - Mian Zhou
human	IHC	BMC Cancer; HLA class II molecule HLA-DRA identifies immuno-hot tumors and predicts the therapeutic response to anti-PD-1 immunotherapy in NSCLC.; Authors - Jie Mei

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