Anticorps Polyclonal de lapin anti-HLA-DRB1

HLA-DRB1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15862-1-AP

Synonymes

Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain

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Galerie de données de validation

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  WB analysis using 15862-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15862-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15862-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 15862-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15862-1-AP (HLA-DRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 15862-1-AP

  IP result of anti-HLA-DRB1 (IP:15862-1-AP, 4ug; Detection:15862-1-AP 1:500) with Raji cells lysate 2000ug.

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  IHC staining of human tonsillitis using 15862-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15862-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15862-1-AP (HLA-DRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 15862-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB1 antibody (15862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules A375, cellules Daudi, tissu splénique humain
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15862-1-AP cible HLA-DRB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-DRB1 Protéine recombinante Ag8405
• Nom complet: major histocompatibility complex, class II, DR beta 1
• Masse moléculaire calculée: 266 aa, 30 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC007920
• Symbole du gène: HLA-DRB1
• Identification du gène (NCBI): 3123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation.; Authors - Semini Geo G
human	WB	Nat Commun; Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes; Authors - Rupert L Mayer
mouse	WB	Heliyon; Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation; Authors - Qian Gao
human	IHC	Front Pharmacol; Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis; Authors - Yanling Wang
mouse	IF	Neuroscience; HLA is a potent immunoinflammatory target in asymptomatic Alzheimer's disease; Authors - Yingwei Zheng

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