Anticorps Polyclonal de lapin anti-HLA-DRB5

HLA-DRB5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 21702-1-AP

Synonymes

DR beta 2, DR beta 5, DR beta-5, DR2-beta-2, Dw2

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Galerie de données de validation

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  WB analysis using 21702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21702-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21702-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21702-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 21702-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 21702-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21702-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21702-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 21702-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21702-1-AP (HLA-DRB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 21702-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRB5 antibody (21702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 21702-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using HLA-DRB5 antibody (21702-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HeLa, cellules HepG2, cellules HL-60, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

21702-1-AP cible HLA-DRB5 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-DRB5 Protéine recombinante Ag16307
• Nom complet: major histocompatibility complex, class II, DR beta 5
• Masse moléculaire calculée: 266 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC009234
• Symbole du gène: HLA-DRB5
• Identification du gène (NCBI): 3127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Open Med (Wars); HLA-DRB5 promotes immune thrombocytopenia via activating CD8+ T cells; Authors - Qidong Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sofia (Verified Customer) (02-03-2025)

Antibody works fine in IHC and IF, in tissue and cells. For Western blot doesn't work as well as in the other techniques.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: brain and microglia

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