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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-HLA-E

HLA-E Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 15008-1-AP

Synonymes

HLA 6.2, HLA E, HLA-6.2, HLAE, MHC class I antigen E



Applications testées

Résultats positifs en WBcellules HUVEC, cellules Raji, cellules THP-1

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15008-1-AP cible HLA-E dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HLA-E Protéine recombinante Ag6703
Nom complet major histocompatibility complex, class I, E
Masse moléculaire calculée 40 kDa
Poids moléculaire observé 40-50 kDa
Numéro d’acquisition GenBankBC002578
Symbole du gène HLA-E
Identification du gène (NCBI) 3133
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-E is a non-classical MHC class I molecule. HLA-E is frequently overexpressed in tumor diseases, transplants and virus-infected cells and represents an immunomodulatory molecule by binding to the receptors CD94/NKG2A, -B and -C on NK and T cells. Due to its immune suppressive features HLA-E expression might represent an important mechanism of tumors to escape immune surveillance.(PMID: 667938; 3375250; 2249951; 27589686)

Protocole

Product Specific Protocols
WB protocol for HLA-E antibody 15008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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