Anticorps Polyclonal de lapin anti-HLTF

• Phare
• Validé par KD/KO

HLTF Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 14286-1-AP

Synonymes

DNA-binding protein/plasminogen activator inhibitor 1 regulator, EC:2.3.2.27, Helicase-like transcription factor, HIP116, HIP116A

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Galerie de données de validation

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  WB analysis of HeLa using 14286-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14286-1-AP (HLTF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14286-1-AP

  IP result of anti-HLTF (IP:14286-1-AP, 4ug; Detection:14286-1-AP 1:500) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 14286-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14286-1-AP (HLTF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14286-1-AP cible HLTF dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLTF Protéine recombinante Ag5596
• Nom complet: helicase-like transcription factor
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC044659
• Symbole du gène: HLTF
• Identification du gène (NCBI): 6596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Helicase-like Transcription Factor (HLTF) is a crucial nuclear protein and member of the SWI/SNF family of chromatin-remodeling complexes, playing a dual role in transcription regulation and DNA damage repair. It functions dually as a regulator of gene expression, utilizing its ATP-dependent helicase activity to modify chromatin structure and facilitate transcription, and as a central effector in the DNA damage response pathway. HLTF is particularly crucial for promoting error-free DNA repair at stalled replication forks via a mechanism called template switching, which prevents the accumulation of mutagenic lesions. HLTF can be detected as 114 kDa.

Publications

Species	Application	Title
mouse	WB	Genes Dev; Break-induced replication promotes fragile telomere formation.; Authors - Zhe Yang
human	WB	Elife; Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.; Authors - Kerrie-Ann McMahon
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao
human	WB,IHC	Int J Oncol; HLTF suppresses the migration and invasion of colorectal cancer cells via TGF‑β/SMAD signaling in vitro.; Authors - Li Liu ; KD Validated
human	CoIP	Oncogenesis; HLTF promotes hepatocellular carcinoma progression by enhancing SRSF1 stability and activating ERK/MAPK pathway; Authors - Yanan Xu ; KD Validated
mouse	WB	Cell Rep; A rewiring of DNA replication mediated by MRE11 exonuclease underlies primed-to-naive cell de-differentiation; Authors - Patricia Ubieto-Capella ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alexandra (Verified Customer) (07-12-2024)

Work perfectly not only with overexpressed protein, but with endogenous ones as well.

• Applications: Western Blot, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

HLTF Antibody Western Blot,Immunofluorescence,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:14286-1-AP)

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