Anticorps Polyclonal de lapin anti-SPP

• Phare
• Validé par KD/KO

SPP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 20416-1-AP

Synonymes

H13, hIMP1, histocompatibility (minor) 13, HM13, IMP 1, IMP1, IMPAS, IMPAS 1, Intramembrane protease 1, MSTP086, Presenilin like protein 3, PSENL3, PSL3, Signal peptide peptidase, SPP

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Galerie de données de validation

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  WB analysis of HEK-293 using 20416-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20416-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20416-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 20416-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20416-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules U2OS, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20416-1-AP cible SPP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPP Protéine recombinante Ag14158
• Nom complet: histocompatibility (minor) 13
• Masse moléculaire calculée: 377 aa, 41 kDa
• Poids moléculaire observé: 45 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC008938
• Symbole du gène: HM13/SPP
• Identification du gène (NCBI): 81502
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Cell Sci; Topology surveillance of the lanosterol demethylase CYP51A1 by Signal Peptide Peptidase; Authors - Nikita Sergejevs ; KD Validated
		PLoS One; Visualizing active enzyme complexes using a photoreactive inhibitor for proximity ligation--application on γ-secretase.; Authors - Schedin-Weiss Sophia S
human	IHC,WB	Cell Death Dis; Histocompatibility Minor 13 (HM13), targeted by miR-760, exerts oncogenic role in breast cancer by suppressing autophagy and activating PI3K-AKT-mTOR pathway; Authors - Haiyan Yang ; KD Validated
human	WB	Front Pharmacol; Pan-cancer analysis suggests histocompatibility minor 13 is an unfavorable prognostic biomarker promoting cell proliferation, migration, and invasion in hepatocellular carcinoma; Authors - Jun Liu ; KD Validated
mouse	IHC	J Exp Clin Cancer Res; miRNA-503 inhibition exerts anticancer effects and reduces tumor growth in mesothelioma; Authors - Miriam Piccioni
human	WB,IHC,IF	Tissue Cell; The role of HM13 expression and its relationship to PI3K/Akt and p53 signaling pathways in colorectal cancer; Authors - Xiao Jin

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