Anticorps Polyclonal de lapin anti-HN1

• Phare
• Validé par KD/KO

HN1 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14914-1-AP

Synonymes

Androgen regulated protein 2, ARM2, HN1, HN1A

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Galerie de données de validation

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  WB analysis of HeLa using 14914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 14914-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14914-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14914-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14914-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14914-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14914-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14914-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14914-1-AP (HN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14914-1-AP

  IP Result of anti-HN1 (IP:14914-1-AP, 4ug; Detection:14914-1-AP 1:1000) with MCF-7 cells lysate 3200ug.

• 

  IHC staining of human ovary tumor using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14914-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14914-1-AP (HN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HepG2, cellules LNCaP, tissu cérébral humain, tissu de côlon de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14914-1-AP cible HN1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HN1 Protéine recombinante Ag6712
• Nom complet: hematological and neurological expressed 1
• Masse moléculaire calculée: 11 kDa, 16 kDa, 19 kDa
• Poids moléculaire observé: 23-30 kDa
• Numéro d’acquisition GenBank: BC001420
• Symbole du gène: HN1
• Identification du gène (NCBI): 51155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HN1 (hematopoietic- and neurologic-expressed sequence 1) is a highly conserved protein that is expressed in developing and regenerating tissues. Overexpression of HN1 has been reported in various tumors like glioma and breast cancer. Hn1 has also been reported as a marker for human ovarian carcinoma and can distinguish epithelial ovarian carcinoma cells from normal ovarian surface epithelial cells. This collective information suggests that HN1 is involved in processes associated with cell proliferation, repair and/or growth.

Publications

Species	Application	Title
human	WB,CoIP	Cancer Lett; HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.; Authors - Zongfu Pan ; KO Validated
human	WB,IHC	Kaohsiung J Med Sci; Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.; Authors - Jia-Jie Chen ; KD Validated
human	WB	Chem Biol Drug Des; Matrine exerts an anti-tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo; Authors - Qiusheng Guo
human	WB,IF	Cancer Lett; Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer; Authors - Zongfu Pan