Anticorps Recombinant de lapin anti-HO-1

• Phare
• Validé par KD/KO

HO-1 Recombinant Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(35)

CloneNo.

2M6

N° de cat : 81281-1-RR

Synonymes

HMOX1, HO-1/HMOX1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1

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Galerie de données de validation

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  WB analysis using 81281-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 81281-1-RR

  WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells.

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  IP experiment of HeLa using 81281-1-RR

  IP result of anti-HO-1 (IP:81281-1-RR, 4ug; Detection:81281-1-RR 1:8000) with HeLa cells lysate 1120 ug.

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  Affinity and Kinetic Characterization of 81281-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81281-1-RR against Human HO-1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules U2OS, tissu splénique de rat
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 33 publications below
• IHC: See 2 publications below

Informations sur le produit

81281-1-RR cible HO-1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HO-1 Protéine recombinante Ag1190
• Nom complet: heme oxygenase (decycling) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001491
• Symbole du gène: HO-1
• Identification du gène (NCBI): 3162
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; Negative regulation of pro-apoptotic AMPK/JNK pathway by itaconate in mice with fulminant liver injury; Authors - Kerui Fan
mouse	WB	Ecotoxicol Environ Saf; Yttrium chloride induces ferroptosis in cardiomyocytes via iron accumulation and triggers cardiac lipid peroxidation and inflammation that cause heart adverse events in mice; Authors - Liang Xiong
mouse	WB	J Agric Food Chem; Lactoferrin Relieves Deoxynivalenol-Induced Oxidative Stress and Inflammatory Response by Modulating the Nrf2/MAPK Pathways in the Liver; Authors - Ping Hu
	WB	Nutr Diabetes; Alantolactone attenuates high-fat diet-induced inflammation and oxidative stress in non-alcoholic fatty liver disease; Authors - Jiong Wang
mouse	WB	Int J Mol Sci; Red Ginseng Improves D-galactose-Induced Premature Ovarian Failure in Mice Based on Network Pharmacology; Authors - Zijing Shang
rat	WB	Eur J Pharmacol; The protective effects of uric acid against myocardial ischemia via the Nrf2 pathway; Authors - Pengqiang Ma

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