Anticorps Polyclonal de lapin anti-HPSE

HPSE Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 24529-1-AP

Synonymes

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 24529-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24529-1-AP (HPSE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 24529-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24529-1-AP (HPSE Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 24529-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24529-1-AP (HPSE Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 24529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 24529-1-AP (HPSE Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 24529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 24529-1-AP (HPSE Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 24529-1-AP

  Immunofluorescent analysis of HeLa cells using 24529-1-AP (HPSE antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 24529-1-AP

  Immunofluorescent analysis of HepG2 cells using 24529-1-AP (HPSE antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

---

Applications testées

• Résultats positifs en WB: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below

Informations sur le produit

24529-1-AP cible HPSE dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HPSE Protéine recombinante Ag21479
• Nom complet: heparanase
• Masse moléculaire calculée: 543 aa, 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC051321
• Symbole du gène: HPSE
• Identification du gène (NCBI): 10855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Publications

Species	Application	Title
human	WB	J Control Release; cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer; Authors - Xiaomei Liang
human	WB,IHC	EMBO Rep; CircHIPK3 sponges miR-558 to suppress heparanase expression in bladder cancer cells.; Authors - Yawei Li
human		Br J Cancer; Shed Syndecan-1 is involved in chemotherapy resistance via the EGFR pathway in colorectal cancer.; Authors - X Wang
rat	IHC	Neural Regen Res; Reducing LncRNA-5657 expression inhibits the brain inflammatory reaction in septic rats.; Authors - Yi-An Zhan
mouse	WB	Diabetes Res Clin Pract; Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.; Authors - Guang Xu
human	IHC, WB	Exp Ther Med; MicroRNA-219a-2-3p modulates the proliferation of thyroid cancer cells via the HPSE/cyclin D1 pathway.; Authors - Chuanjia Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Fabio Henrique (Verified Customer) (01-17-2019)

Figure 1. IF of HPSE (red) and CD68 (green) in 3D coculture of C4-2B and primary human macrophages. C4-2B and primary human macrophages were encapsulated in a 3D hydrogel, composed of hyaluronic acid and collagen type 1, and cocultured for 5 days. On day 5, cells were fixed in 4% paraformaldehyde for 10 minutes, washed with PBSTritonX 0.3% for 3x 5min and blocked in PBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-HPSE was used at 1:100 in blocking buffer, overnight incubation at 4*C. Secondary antibodies AlexaFluor 488 and 568 were used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: HPSE (red) and CD68 (green) in Grade 3 Endometrial cancer tissue (Frozen). Staining was performed as described above, except secondary antibodies were used at 1:1000. This antibody did not perform well in WB (data not shown).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: C4-2B and Primary Macrophages; Grade 3 Endometrial Cancer tissue (Frozen)

HPSE Antibody Immunofluorescence, validation (1:100 dilution) in C4-2B and Primary Macrophages; Grade 3 Endometrial Cancer tissue (Frozen) (Cat no:24529-1-AP)