Anticorps Polyclonal de lapin anti-HSD17B2

• Phare
• Validé par KD/KO

HSD17B2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 10978-1-AP

Synonymes

17 beta HSD 2, 17βHSD2, 20 alpha HSD, E2DH, EDH17B2, HSD17, HSD17B2, SDR9C2

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Galerie de données de validation

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  WB analysis using 10978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10978-1-AP

  WB result of HSD17B2 antibody (10978-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B2 transfected HepG2 cells.

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  WB analysis of MCF-7 using 10978-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10978-1-AP (HSD17B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary tumor using 10978-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10978-1-AP (HSD17B2 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 21 publications below

Informations sur le produit

10978-1-AP cible HSD17B2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSD17B2 Protéine recombinante Ag1411
• Nom complet: hydroxysteroid (17-beta) dehydrogenase 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC009581
• Symbole du gène: HSD17B2
• Identification du gène (NCBI): 3294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSD17B2(Estradiol 17-beta-dehydrogenase 2) is also named as 17-beta-HSD 2,20-alpha-HSD,EDH17B2 and belongs to the short-chain dehydrogenases/reductases (SDR) family.Based on the pivotal role of HSD17B2 gene product in the estrogen metabolism pathway, it may be considered as a putative candidate gene that could possibly explain a fraction of the remaining familial breast cancer risk and it is unlikely to play a role as a high penetrance gene in breast cancer predisposition(PMID:18372405).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	IHC	J Clin Endocrinol Metab; MUC1 as a discriminator between endometrium from fertile and infertile patients with PCOS and endometriosis.; Authors - Margarit L L
human	IHC	Br J Cancer; In breast cancer subtypes steroid sulfatase (STS) is associated with less aggressive tumour characteristics.; Authors - Keely M McNamara
human	WB,IHC	Fertil Steril; Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.; Authors - Miao Sun
human	WB,IHC	Endocr Relat Cancer; PMID: 20228125; Authors - Takagi Kiyoshi K
rat	WB	Ecotoxicol Environ Saf; Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression.; Authors - Changjiang Liu