Anticorps Polyclonal de lapin anti-HSDL2

• Phare
• Validé par KD/KO

HSDL2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15631-1-AP

Synonymes

C9orf99, HSDL2, SDR13C1

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Galerie de données de validation

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  WB analysis using 15631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15631-1-AP (HSDL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15631-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15631-1-AP (HSDL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15631-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSDL2 antibody (15631-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

15631-1-AP cible HSDL2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSDL2 Protéine recombinante Ag7987
• Nom complet: hydroxysteroid dehydrogenase like 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC004331
• Symbole du gène: HSDL2
• Identification du gène (NCBI): 84263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human hydroxysteroid dehydrogenase-like 2 (HSDL2) is a characterized SDR gene that not only catalyses the oxidation and reduction of multiple substrates but also regulates different metabolic and signalling pathways. Accumulating evidences suggest that HSDL2 play an important role in cancer progression. HSDL2 has promoting effects on tumor progression in papillary thyroid cancer, bladder cancer, ovarian cancer, and glioma, but has suppressing effects in cholangiocarcinoma . ( PMID: 31372054, PMID: 32211805)

Publications

Species	Application	Title
human	WB	Proteomics; Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.; Authors - Songlin Zeng
human	IF	Cancer Manag Res; Ectopic expression of HSDL2 is related to cell proliferation and prognosis in breast cancer.; Authors - Bing Dong
human	IHC,WB	Onco Targets Ther; HSDL2 Acts as a Promoter in Pancreatic Cancer by Regulating Cell Proliferation and Lipid Metabolism.; Authors - Anna Han ; KD Validated
human	WB,FC	Chin Med; Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression.; Authors - Wen-Bei Liu ; KD Validated
human	IHC	Nan Fang Yi Ke Da Xue Xue Bao; [HSDL2 overexpression promotes rectal cancer progression by regulating cancer cell cycle and promoting cell proliferation]; Authors - Y Cheng ; KD Validated
human	WB,IHC	BMC Cancer; MiR-26a-5p regulates proliferation, apoptosis, migration and invasion via inhibiting hydroxysteroid dehydrogenase like-2 in cervical cancer cell; Authors - Ming Li