Anticorps Polyclonal de lapin anti-HSPA1B

HSPA1B Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 25405-1-AP

Synonymes

HSP70, HSP72

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Galerie de données de validation

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  WB analysis using 25405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25405-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25405-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25405-1-AP

  IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:5000) with HEK-293 cells lysate 2320 ug.

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  IP experiment of K-562 using 25405-1-AP

  IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IHC staining of human stomach using 25405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25405-1-AP (HSPA1B antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25405-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25405-1-AP (HSP70 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293, cellules K-562
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

25405-1-AP cible HSPA1B dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA1B Protéine recombinante Ag13700
• Nom complet: heat shock 70kDa protein 1B
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC057397
• Symbole du gène: HSP70
• Identification du gène (NCBI): 3304
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1. What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

2. FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

a. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Publications

Species	Application	Title
human	WB	Nat Commun; Feedback inhibition of cAMP effector signaling by a chaperone-assisted ubiquitin system.; Authors - Laura Rinaldi
mouse	WB	Hum Mol Genet; Loss of Ten-Eleven Translocation 2 induces cardiac hypertrophy and fibrosis through modulating ERK signaling pathway.; Authors - Huikang Tao
human	WB	Oncotarget; Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.; Authors - Liyuan Zhang
human	WB	Am J Physiol Renal Physiol; Exosomes derived from TREM-2 knocked-out macrophages alleviated renal fibrosis via HSPa1b/AKT pathway; Authors - Zihao Xiao
human	WB	Cell Death Dis; Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma; Authors - Tianyin Ma
rat	WB	Mol Med; HSPA1A inhibits pyroptosis and neuroinflammation after spinal cord injury via DUSP1 inhibition of the MAPK signaling pathway; Authors - Xuegang He

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