Anticorps Polyclonal de lapin anti-HSP90

• Phare
• Validé par KD/KO

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(37)

Avis (2)

N° de cat : 11405-1-AP

Synonymes

HSP90AB1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HSP90B

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Galerie de données de validation

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11405-1-AP

  WB result of HSP90AB1 antibody (11405-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11405-1-AP

  IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human pancreas cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 11405-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90AB1 antibody (11405-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11405-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP90AB1 Polyclonal antibody (11405-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 34 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

11405-1-AP cible HSP90 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP90 Protéine recombinante Ag1947
• Nom complet: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Masse moléculaire calculée: 723 aa, 83 kDa
• Poids moléculaire observé: 83-90 kDa
• Numéro d’acquisition GenBank: BC014485
• Symbole du gène: HSP90AB1
• Identification du gène (NCBI): 3326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Publications

Species	Application	Title
human	WB,IP	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
pig	WB	Autophagy; Induction of autophagy and suppression of type I IFN secretion by CSFV.; Authors - Baoming Xie
	WB	Cell Rep; CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons; Authors - Carissa L Sirois
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
human	WB	Anal Chem; Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.; Authors - Xiaolei Zhang
sheep	IF	PLoS Pathog; A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.; Authors - Thomas Labadie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maria (Verified Customer) (07-06-2022)

Very good antibody, we achieved good results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Fibroblasts - human

FH

Kyosuke (Verified Customer) (06-12-2019)

it works good for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

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