Anticorps Polyclonal de lapin anti-HSP70

• Phare
• Validé par KD/KO

HSP70 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(328)

Avis (6)

N° de cat : 10995-1-AP

Synonymes

HSPA1A, Heat shock 70 kDa protein 1A, heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70.1

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Galerie de données de validation

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  WB analysis using 10995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine exosomes using 10995-1-AP

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10995-1-AP

  IP result of anti-HSP70 (IP:10995-1-AP, 3ug; Detection:10995-1-AP 1:1000) with mouse brain tissue lysate 3600ug.

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  IHC staining of human breast cancer using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10995-1-AP (HSP70 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10995-1-AP (HSP70 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse skin using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10995-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10995-1-AP (HSP70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10995-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HSP70 antibody (10995-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 10995-1-AP

  Immunofluorescent analysis of HepG2 cells, using HSPA1A antibody 10995-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HeLa using 10995-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP70 Polyclonal antibody (10995-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, cerveau de souris, rein de rat, rein de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cutané de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 302 publications below
• IHC: See 13 publications below
• IF: See 26 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 5 publications below

Informations sur le produit

10995-1-AP cible HSP70 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, Lapin, poisson-zèbre, poulet, souris, oyster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP70 Protéine recombinante Ag1446
• Nom complet: heat shock 70kDa protein 1A
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC009322
• Symbole du gène: HSP70
• Identification du gène (NCBI): 3303
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1. What is Hsp70/HSP1A?

   HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

2. FAQs for Hsp70

   a. I cannot detect Hsp70 by western blotting

   HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

   b. What loading control can I use for cellular stress experiments with Hsp70?

   Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Publications

Species	Application	Title
human	WB	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	WB	Nat Cell Biol; Caspase-2 is a condensate-mediated deubiquitinase in protein quality control; Authors - Yingwei Ge
human	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo ; KO Validated
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
mouse	IF	Adv Sci (Weinh); Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy; Authors - Doudou Lei

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PK (Verified Customer) (08-14-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: ac16

HSP70 Antibody Western Blot validation (1000 dilution) in ac16 (Cat no:10995-1-AP)

FH

Scott (Verified Customer) (05-22-2024)

20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373.

• Applications: Western Blot
• Primary Antibody Dilution: 1 : 5000
• Cell Tissue Type: TDP-Q331K Cortex and Spinal Cord Tissue Lysate

HSP70 Antibody Western Blot validation (1 : 5000 dilution) in TDP-Q331K Cortex and Spinal Cord Tissue Lysate (Cat no:10995-1-AP)

FH

Nin (Verified Customer) (01-28-2023)

A very good antibody to probe HSP70 by WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

HSP70 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:10995-1-AP)

FH

Tsimafei (Verified Customer) (12-04-2022)

20µg of the total protein were used. Incubation was done for 2h at room temperature

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: mESCs

HSP70 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:10995-1-AP)

FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: ARPE-19

HSP70 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:10995-1-AP)

FH

Luxi (Verified Customer) (11-23-2020)

This antibody works well in my tissue. It binds to the nuclear and cytosol Hsp70s.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:60
• Cell Tissue Type: embryonic cells

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