Anticorps Polyclonal de lapin anti-HSPA1B

HSPA1B Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25405-1-PBS

Synonymes

HSP72

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Galerie de données de validation

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  WB analysis using 25405-1-AP (same clone as 25405-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 25405-1-AP (same clone as 25405-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 25405-1-AP (same clone as 25405-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25405-1-AP (HSP70 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 25405-1-AP (same clone as 25405-1-PBS)

  IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:5000) with HEK-293 cells lysate 2320 ug. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 25405-1-AP (same clone as 25405-1-PBS)

  IP result of anti-HSP70 (IP:25405-1-AP, 4ug; Detection:25405-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 25405-1-AP (same clone as 25405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25405-1-AP (HSP70 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 25405-1-AP (same clone as 25405-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25405-1-AP (HSPA1B antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25405-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25405-1-PBS cible HSPA1B dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA1B Protéine recombinante Ag13700
• Nom complet: heat shock 70kDa protein 1B
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC057397
• Symbole du gène: HSP70
• Identification du gène (NCBI): 3304
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

1. What is Hsp70/HSP1A?

HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

2. FAQs for Hsp70

a. I cannot detect Hsp70 by western blotting

HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

a. What loading control can I use for cellular stress experiments with Hsp70?

Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

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