Anticorps Polyclonal de lapin anti-HSPA6

HSPA6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 13616-1-AP

Synonymes

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

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Galerie de données de validation

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  WB analysis using 13616-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13616-1-AP (HSPA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13616-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13616-1-AP (HSPA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 13616-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13616-1-AP (HSPA6 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules MCF-7, cellules U-937
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13616-1-AP cible HSPA6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPA6 Protéine recombinante Ag4522
• Nom complet: heat shock 70kDa protein 6 (HSP70B')
• Masse moléculaire calculée: 643 aa, 71 kDa
• Poids moléculaire observé: 68-71 kDa
• Numéro d’acquisition GenBank: BC035665
• Symbole du gène: HSPA6
• Identification du gène (NCBI): 3310
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)

Publications

Species	Application	Title
rat	WB,IF	J Cell Mol Med; ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo; Authors - Rui Guo
human	WB	Am J Cancer Res; The stabilization of PD-L1 by the endoplasmic reticulum stress protein GRP78 in triple-negative breast cancer.; Authors - Cheng-Wei Chou
pig	WB	Biochim Biophys Acta Mol Cell Res; Generation of a pHSPA6 gene-based multifunctional live cell sensor.; Authors - Zicong Xie
pig	WB	Am J Physiol Heart Circ Physiol; Molecular mechanisms mediating preconditioning following chronic ischemia differ from those in classical second window.; Authors - Depre Christophe C
human	WB	Evid Based Complement Alternat Med; ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling.; Authors - Junyi Tang
human	WB	Biosci Biotechnol Biochem; ARHGEF10L expression regulates cell proliferation and migration in gastric tumorigenesis.; Authors - Da-Wei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Laureano (Verified Customer) (12-26-2019)

The antibody gave a very clean band without background

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mda-mb-231