• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GRP75

GRP75 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(53)

N° de cat : 14887-1-AP

Synonymes

HSPA9, 75 kDa glucose-regulated protein, CSA, GRP-75, Heat shock 70 kDa protein 9



Applications testées

Résultats positifs en WBcellules HT-29, cellules A549, cellules Jurkat, cellules NIH/3T3, foie de souris, tissu testiculaire de souris
Résultats positifs en IPtissu cérébral de souris, cellules NIH/3T3
Résultats positifs en IHChuman normal colon,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,
Résultats positifs en FC (Intra)cellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14887-1-AP cible GRP75 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, rat, souris

Réactivité Humain, poisson-zèbre, rat, souris
Réactivité citéerat, Humain, poisson-zèbre, poulet, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GRP75 Protéine recombinante Ag6673
Nom complet heat shock 70kDa protein 9 (mortalin)
Masse moléculaire calculée 74 kDa
Poids moléculaire observé 70-75 kDa
Numéro d’acquisition GenBankBC000478
Symbole du gène GRP75
Identification du gène (NCBI) 3313
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

Protocole

Product Specific Protocols
WB protocol for GRP75 antibody 14887-1-APDownload protocol
IHC protocol for GRP75 antibody 14887-1-APDownload protocol
IF protocol for GRP75 antibody 14887-1-APDownload protocol
IP protocol for GRP75 antibody 14887-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism

Authors - Wang Jiahui
  • KD Validated
humanWB

Cell Metab

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Authors - Ricarda Richter-Dennerlein
humanWB

Nat Commun

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Authors - Chun-Shik Shin
mouse

EMBO Mol Med

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Authors - Masayuki Ide
mouseWB

J Cell Biol

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

Authors - Anne Korwitz
ratWB

Cell Death Dis

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats

Authors - Yajie Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Lenie (Verified Customer) (07-18-2025)

IF worked. WB did not on zebrafish tissue and human patient. Could you perhaps provide me with another protocol?

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/5000 (WB), 1/200 (IF)
  • Cell Tissue Type: Zebrafish
GRP75 Antibody Western Blot,Immunofluorescence validation (1/5000 (WB), 1/200 (IF) dilution) in Zebrafish (Cat no:14887-1-AP)
FH

Anastasia (Verified Customer) (08-07-2024)

Deyolked zebrafish embryos

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Zebrafish embryos
GRP75 Antibody Western Blot validation (1:1000 dilution) in Zebrafish embryos (Cat no:14887-1-AP)
FH

Christina (Verified Customer) (11-14-2023)

I used this antibody for HeLa extracts in Western Blot and worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HeLa cells
FH

Ning (Verified Customer) (02-27-2023)

Specifically probe endogenous GRP75 with a right MW.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
GRP75 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14887-1-AP)
FH

Staci (Verified Customer) (02-02-2023)

Antibody works very well with GRP75. Very little non-specific binding on immunoblot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse Islet protein lysates
{{ptg:RelatedPrimaryAntibodies}}