Anticorps Polyclonal de lapin anti-GRP75

• Phare
• Validé par KD/KO

GRP75 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (3)

N° de cat : 14887-1-AP

Synonymes

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

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Galerie de données de validation

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  WB analysis using 14887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 cells using 14887-1-AP

  A549 cells (shcontrol and shRNA of GRP75)were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 antibody) at dilution of 1:500.

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  WB analysis of mouse testis using 14887-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14887-1-AP (GRP75 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14887-1-AP

  IP Result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:2000) with mouse brain tissue lysate 3680ug.

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  IP experiment of NIH/3T3 using 14887-1-AP

  IP Result of anti-GRP75 (IP:14887-1-AP, 4ug; Detection:14887-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human breast cancer using 14887-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14887-1-AP (GRP75 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14887-1-AP

  Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 14887-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GRP75 antibody (14887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 14887-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug GRP75 antibody (14887-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules A549, cellules Jurkat, cellules NIH/3T3, foie de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

14887-1-AP cible GRP75 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRP75 Protéine recombinante Ag6673
• Nom complet: heat shock 70kDa protein 9 (mortalin)
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 74-75 kDa
• Numéro d’acquisition GenBank: BC000478
• Symbole du gène: HSPA9
• Identification du gène (NCBI): 3313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

Publications

Species	Application	Title
human	WB	Cell Metab; DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.; Authors - Ricarda Richter-Dennerlein
human	WB	Nat Commun; LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.; Authors - Chun-Shik Shin
mouse		EMBO Mol Med; Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.; Authors - Masayuki Ide
mouse	WB	J Cell Biol; Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.; Authors - Anne Korwitz
rat	WB	Cell Death Dis; Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats; Authors - Yajie Wang
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christina (Verified Customer) (11-14-2023)

I used this antibody for HeLa extracts in Western Blot and worked perfectly.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HeLa cells

FH

Ning (Verified Customer) (02-27-2023)

Specifically probe endogenous GRP75 with a right MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

GRP75 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:14887-1-AP)

FH

Staci (Verified Customer) (02-02-2023)

Antibody works very well with GRP75. Very little non-specific binding on immunoblot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Islet protein lysates

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