Anticorps Polyclonal de lapin anti-HSPB8

HSPB8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 15287-1-AP

Synonymes

E2-induced gene 1 protein, E2IG1, DHMN2, CRYAC, CMT2L

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Galerie de données de validation

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  WB analysis using 15287-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 15287-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15287-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15287-1-AP (HSPB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15287-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15287-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 15287-1-AP (HSPB8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules HEK-293, cellules SGC-7901, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15287-1-AP cible HSPB8 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPB8 Protéine recombinante Ag7456
• Nom complet: heat shock 22kDa protein 8
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC002673
• Symbole du gène: HSPB8
• Identification du gène (NCBI): 26353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Heat Shock Protein B8 (HSPB8), also named small stress protein-like protein (sHSP22), protein kinase H11(H11), E2-induced gene 1 protein (E2IG1), or alpha-crystallin C chain (CRYAC), is a small chaperone involved in chaperone-assisted selective autophagy (CASA) (PMID: 33562660). HSPB8 is expressed at relatively high levels in the heart and the skeletal muscle tissue. HSPB8 is composed of 196 amino acids with an apparent molecular mass of 21.6 kDa (PMID: 20570967).

Publications

Species	Application	Title
human	WB, IF	Nat Commun; Cardiomyocyte contractile impairment in heart failure results from reduced BAG3-mediated sarcomeric protein turnover.; Authors - Thomas G Martin
mouse	IHC	J Neurosci; The α crystallin domain of small heat shock protein b8 (Hspb8) acts as survival and differentiation factor in adult hippocampal neurogenesis.; Authors - Ramírez-Rodríguez Gerardo G
human	WB	J Am Heart Assoc; Reduction of Filamin C Results in Altered Proteostasis, Cardiomyopathy, and Arrhythmias; Authors - Joyce C Ohiri
mouse	WB	J Cell Biochem; Pharmacological inhibition of BAG3-HSP70 with the proposed cancer therapeutic JG-98 is toxic for cardiomyocytes.; Authors - Thomas G Martin
mouse,human	CoIP	Genes (Basel); BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation.; Authors - Hongtao Diao
human	WB	Cell Rep; Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR.; Authors - Dawid Krokowski

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