Anticorps Polyclonal de lapin anti-HSPBP1

• Phare
• Validé par KD/KO

HSPBP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10211-1-AP

Synonymes

FES1, Hsp70 binding protein 1, Hsp70 binding protein 2, Hsp70 interacting protein 1, Hsp70 interacting protein 2, HSPBP, HSPBP1, HspBP2

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Galerie de données de validation

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  WB analysis using 10211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10211-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10211-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10211-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10211-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10211-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 10211-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10211-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10211-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10211-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10211-1-AP (HSPBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10211-1-AP (HSPBP1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10211-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10211-1-AP (HSPBP1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HeLa using 10211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSPBP1 antibody (10211-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules MCF-7, tissu cardiaque humain, tissu cérébral de souris, tissu de muscle squelettique humain, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10211-1-AP cible HSPBP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSPBP1 Protéine recombinante Ag0282
• Nom complet: HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC002373
• Symbole du gène: HSPBP1
• Identification du gène (NCBI): 23640
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Hsp70-interacting protein (HSPBP1)is a protein of approximately 40 kilodaltons detected in cell extracts. Its mRNA is present in all tissues analyzed but is most abundant in heart and skeletal muscle. HSPBP1 binds the ATPase domain of Hsp70 and inhibits approximately 90% of the Hsp40-activated Hsp70 ATPase activity by preventing ATP binding to Hsp70.

Publications

Species	Application	Title
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
human	WB	Mol Immunol; HSPBP1 facilitates cellular RLR-mediated antiviral response by inhibiting the K48-linked ubiquitination of RIG-I.; Authors - Ya-Xian Yang ; KD Validated