Anticorps Polyclonal de lapin anti-HSP60

• Phare
• Validé par KD/KO

HSP60 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

111

N° de cat : 15282-1-AP

Synonymes

HSP 60, HSP60, HSP65, HSPD1

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Galerie de données de validation

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  WB analysis using 15282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15282-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15282-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15282-1-AP (HSP60 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15282-1-AP

  IP result of anti-HSP60(IP:15282-1-AP, 4ug; Detection:15282-1-AP 1:10000) with HEK-293 cells lysate 1800 ug.

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  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15282-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15282-1-AP (HSP60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15282-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP60 antibody (15282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

• 

  FC experiment of HepG2 using 15282-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP60 (15282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules NIH/3T3, cœur de rat, cœur de souris, HeLa
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 91 publications below
• IHC: See 6 publications below
• IF: See 23 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15282-1-AP cible HSP60 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, levure, porc, poulet, riz, souris, duck, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP60 Protéine recombinante Ag7401
• Nom complet: heat shock 60kDa protein 1 (chaperonin)
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC003030
• Symbole du gène: HSPD1
• Identification du gène (NCBI): 3329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Publications

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
mouse	WB	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
mouse	WB	J Hazard Mater; Long-term effect of PBDE-99 prenatal exposure on spermatogenic injuries via the dysregulation of autophagy; Authors - Jun Wu
human	WB	Nat Commun; Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response.; Authors - Wenjun Wang
mouse	WB,IF	Nat Commun; Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells.; Authors - Gustavo Della-Flora Nunes
human	WB	Nat Commun; EGFL9 promotes breast cancer metastasis by inducing cMET activation and metabolic reprogramming.; Authors - Fanyan Meng

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