Anticorps Monoclonal anti-HTRA1

HTRA1 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11G12

N° de cat : CL488-68053

Synonymes

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-68053

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HTRA1 antibody (CL488-68053, Clone: 1B11G12 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-68053

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Htra1 Monoclonal Antibody (CL488-68053, Clone:1B11G12)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-68053 cible HTRA1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HTRA1 Protéine recombinante Ag31204
• Nom complet: HtrA serine peptidase 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: NM_002775
• Symbole du gène: HTRA1
• Identification du gène (NCBI): 5654
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

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