Anticorps Polyclonal de lapin anti-HTRA2

• Phare
• Validé par KD/KO

HTRA2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 15775-1-AP

Synonymes

HtrA serine peptidase 2, HTRA2, OMI, PARK13, PRSS25, Serine protease 25, Serine proteinase OMI

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Galerie de données de validation

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  WB analysis of HeLa using 15775-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15775-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15775-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15775-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15775-1-AP (HTRA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 15775-1-AP

  IP Result of anti-HTRA2 (IP:15775-1-AP, 4ug; Detection:15775-1-AP 1:500) with Raji cells lysate 2000ug.

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  IHC staining of human kidney using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15775-1-AP (HTRA2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human thyroid using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 15775-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15775-1-AP (HTRA2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7, cellules HEK-293, cellules Jurkat, cellules MCF-7, cellules Neuro-2a, cellules Raji, cellules SH-SY5Y, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu rénal humain, tissu de côlon humain, tissu thyroïdien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

15775-1-AP cible HTRA2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HTRA2 Protéine recombinante Ag8455
• Nom complet: HtrA serine peptidase 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000096
• Symbole du gène: HTRA2
• Identification du gène (NCBI): 27429
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
mouse	WB	Int J Mol Sci; The Mechanism of Mitochondrial Injury in Alpha-1 Antitrypsin Deficiency Mediated Liver Disease.; Authors - Nazli Khodayari
		Mol Neurobiol; Mitochondrial Proteome Changes Correlating with β-Amyloid Accumulation.; Authors - Katalin Völgyi
human	WB	Hum Mol Genet; Multi-OMICS study of a CHCHD10 variant causing ALS demonstrates metabolic rewiring and activation of endoplasmic reticulum and mitochondrial unfolded protein responses.; Authors - Isabella R Straub
human	WB	Cancer Sci; Establishment of MAGEC2-knockout cells and functional investigation of MAGEC2 in tumor cells.; Authors - Jingjing Wang
human	IHC	Cancer Biomark; Role of Smac, survivin, XIAP, and Omi/HtrA2 proteins in determining the chemotherapeutic response of patients with cervical cancer treated with neoadjuvant chemotherapy; Authors - Tianyi Ye