Anticorps Monoclonal anti-Heavy chain of Rabbit IgG

Heavy chain of Rabbit IgG Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Lapin et plus (1)

Applications

WB, IP

Conjugaison

HRP

Publications

2

CloneNo.

1C9A5

N° de cat : HRP-66467

Synonymes

Heavy Chain, Heavy Chain of Rabbit IgG, Rabbit IgG

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Galerie de données de validation

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  WB analysis using HRP-66467

  Various serum/plasma lysate were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using HRP-66467

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. HRP-66467 (Heavy chain of Rabbit IgG antibody) as secondary antibody with dilution 1:15000. .

• 

  WB analysis of HeLa using HRP-66467

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-66467 (Heavy chain of Rabbit IgG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu sérique de lapin

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

HRP-66467 cible Heavy chain of Rabbit IgG dans les applications de WB, IP et montre une réactivité avec des échantillons Lapin

• Réactivité: Lapin
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine
• Nom complet: Heavy chain of Rabbit IgG
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Publications

Species	Application	Title
	WB	Transl Res; A key role for NLRP3 signaling in preterm labor and birth driven by the alarmin S100B; Authors - Jose Galaz
mouse	IP	FEBS J; NDRG2 alleviates photoreceptor apoptosis by regulating the STAT3/TIMP3/MMP pathway in mice with retinal degenerative disease; Authors - Tao Chen