Anticorps Polyclonal de lapin anti-Human IgG

Human IgG Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(26)

Avis (1)

N° de cat : 10284-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of human plasma using 10284-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 10284-1-AP (Human IgG antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10284-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10284-1-AP (Human IgG antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 10284-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgG antibody (10284-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 6 publications below
• RIP: See 2 publications below

Informations sur le produit

10284-1-AP cible Human IgG dans les applications de WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Human IgG
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 25 kDa, 50 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

This rabbit poly clonal antibody recognizes the human IgG.

Publications

Species	Application	Title
	WB	Sci Adv; Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.; Authors - Long Qin
		Int Immunopharmacol; LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.; Authors - Zhi-Min Wang
	WB	Arch Biochem Biophys; Fructose-derived glycation and immune function: Effects on antigen binding in human IgG and lymphocytes; Authors - Feryal Akay
		Front Bioeng Biotechnol; Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.; Authors - Rui-Rui Guo
		Biosensors (Basel); Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.; Authors - Yijian Huang
	CoIP	Int J Oral Sci; FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.; Authors - Xinyuan Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fatima (Verified Customer) (12-11-2018)

• Applications: Flow Cytometry,

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