Anticorps Monoclonal anti-IA-2/PTPRN

IA-2/PTPRN Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D3F5

N° de cat : CL488-66045

Synonymes

IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-66045

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66045 (IA-2/PTPRN antibody) at dilution of 1:100.

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  FC experiment of HEK-293 using CL488-66045

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IA-2/PTPRN (CL488-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Y79 using CL488-66045

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IA-2/PTPRN (CL488-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HEK-293, cellules Y79

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66045 cible IA-2/PTPRN dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IA-2/PTPRN Protéine recombinante Ag17909
• Nom complet: protein tyrosine phosphatase, receptor type, N
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC007713
• Symbole du gène: IA-2/PTPRN
• Identification du gène (NCBI): 5798
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

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