Anticorps Recombinant de lapin anti-IARS2

IARS2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242219C5

N° de cat : 84764-3-RR

Synonymes

242219C5, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial

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Galerie de données de validation

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  WB analysis using 84764-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84764-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84764-3-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of Human Hepatocellular cancer using 84764-3-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular cancer using 84764-3-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84764-3-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84764-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 84764-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-3-RR, Clone: 242219C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 84764-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IARS2 Recombinant antibody (84764-3-RR, Clone:242219C5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84764-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84764-3-RR against Human IARS2 were performed. The affinity constant is 0.469 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7, tissu cardiaque de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: Human Hepatocellular cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84764-3-RR cible IARS2 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IARS2 Protéine recombinante Ag10696
• Nom complet: isoleucyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 1012 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC010218
• Symbole du gène: IARS2
• Identification du gène (NCBI): 55699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

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