Anticorps Monoclonal anti-IBA1

IBA1 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

1C6A10

N° de cat : CL647-66827

Synonymes

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Galerie de données de validation

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  FC experiment of THP-1 using CL647-66827

  1x10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated IBA1 Monoclonal antibody (CL647-66827, Clone:1C6A10)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG2a isotype control (11A1B2) (CL647-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules THP-1
• Résultats positifs en cytométrie: cellules THP-1

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-66827 cible IBA1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IBA1 Protéine recombinante Ag28236
• Nom complet: allograft inflammatory factor 1
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC009474
• Symbole du gène: IBA1
• Identification du gène (NCBI): 199
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.

Publications

Species	Application	Title
mouse		Acta Pharm Sin B; Drofenine as a Kv2.1 inhibitor alleviated AD-like pathology in mice through Aβ/Kv2.1/microglial NLRP3/neuronal Tau axis; Authors - Jian Lu

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