Anticorps Monoclonal anti-IDE

• Phare
• Validé par KD/KO

IDE Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

3E10G1

N° de cat : 67106-1-Ig

Synonymes

Insulin-degrading enzyme, Insulinase, Insulin protease, EC:3.4.24.56, Abeta-degrading protease

---

Galerie de données de validation

• 

  WB analysis using 67106-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67106-1-Ig

  WB result of IDE antibody (67106-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells.

• 

  WB analysis of RAW 264.7 using 67106-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67106-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67106-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67106-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67106-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67106-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67106-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67106-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 67106-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 67106-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67106-1-Ig (IDE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules A431, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HT-1080, cellules Jurkat, cellules LNCaP, cellules MCF-7, cellules NCCIT, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

67106-1-Ig cible IDE dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IDE Protéine recombinante Ag17161
• Nom complet: Abeta degrading protease
• Masse moléculaire calculée: 1019 aa, 118 kDa
• Poids moléculaire observé: 100-120 kDa
• Numéro d’acquisition GenBank: BC096336
• Symbole du gène: IDE
• Identification du gène (NCBI): 3416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).

Publications

Species	Application	Title
human,mouse	WB	Aging Cell; Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease; Authors - Guoxin Zhang
mouse	WB	Mol Nutr Food Res; Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway; Authors - Qian Su
mouse	WB	Mol Med; Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head; Authors - Tao Yuan
mouse	WB	Phytomedicine; Osmundacetone ameliorates Alzheimer's-like pathologies by inhibiting β-amyloid fibrillation, oxidative damage and neuroinflammation in APP/PS1 transgenic mice; Authors - Ling-Xiao Zhao
human	WB	Phytomedicine; Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway; Authors - Xiao Liu

{{ptg:RelatedPrimaryAntibodies}}