Anticorps Polyclonal de lapin anti-IDH1

• Phare
• Validé par KD/KO

IDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(59)

Avis (1)

N° de cat : 12332-1-AP

Synonymes

Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH, IDH 1

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Galerie de données de validation

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  WB analysis using 12332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12332-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 12332-1-AP

  WB result of IDH1 antibody (12332-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and IDH1 knockout HAP1 cells.

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  WB analysis of rat liver using 12332-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12332-1-AP (IDH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12332-1-AP

  IP result of anti-IDH1 (IP:12332-1-AP, 4ug; Detection:12332-1-AP 1:500) with HepG2 cells lysate 1880 ug.

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  IHC staining of human liver cancer using 12332-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12332-1-AP (IDH1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human intrahepatic cholangiocarcinoma using 12332-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12332-1-AP (IDH1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12332-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH1 antibody (12332-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 52 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below

Informations sur le produit

12332-1-AP cible IDH1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDH1 Protéine recombinante Ag2986
• Nom complet: isocitrate dehydrogenase 1 (NADP+), soluble
• Masse moléculaire calculée: 414 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC012846
• Symbole du gène: IDH1
• Identification du gène (NCBI): 3417
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

Publications

Species	Application	Title
mouse	WB	Nature; α-Ketoglutarate links p53 to cell fate during tumour suppression.; Authors - John P Morris ; KD Validated
human	WB,IHC	Nature; IDH mutation impairs histone demethylation and results in a block to cell differentiation.; Authors - Lu Chao C
human	WB	Nature; Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.; Authors - Dang Lenny L
human	WB	Nature; Quantitative flux analysis reveals folate-dependent NADPH production.; Authors - Jing Fan
human	WB	Cancer Cell; Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.; Authors - Maria E Figueroa
human	WB	Cell Stem Cell; PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.; Authors - Zhexin Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Umut (Verified Customer) (07-25-2023)

The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U87MG

IDH1 Antibody Western Blot validation (1:1000 dilution) in U87MG (Cat no:12332-1-AP)

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