Anticorps Polyclonal de lapin anti-IDO1

• Phare
• Validé par KD/KO

IDO1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

55

Avis (2)

N° de cat : 13268-1-AP

Synonymes

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase

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Galerie de données de validation

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  WB analysis using 13268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13268-1-AP (IDO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13268-1-AP

  Human placenta were subjected to SDS PAGE followed by western blot with 13268-1-AP (IDO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13268-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13268-1-AP (IDO1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13268-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13268-1-AP (IDO1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HeLa traitées à l'IFN gamma
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 44 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Informations sur le produit

13268-1-AP cible IDO1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IDO1 Protéine recombinante Ag3953
• Nom complet: indoleamine 2,3-dioxygenase 1
• Masse moléculaire calculée: 403 aa, 45 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC027882
• Symbole du gène: IDO1
• Identification du gène (NCBI): 3620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

Publications

Species	Application	Title
human	WB	Hepatology; Indoleamine-2, 3-dioxygenase as an effector and an indicator of protective immune responses in patients with acute hepatitis B.; Authors - Sachiyo Yoshio ; KO Validated
mouse	WB	Acta Biomater; Blockage of the IDO1 pathway by charge-switchable nanoparticles amplifies immunogenic cell death for enhanced cancer immunotherapy.; Authors - Menghao Shi
mouse	WB	ACS Appl Mater Interfaces; Mitigation Effects of Selenium Nanoparticles on Depression-Like Behavior Induced by Fluoride in Mice via the JAK2-STAT3 Pathway.; Authors - Jiarong Yang
human	WB	J Immunother Cancer; GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.; Authors - Jin-Li Wei
human	WB	J Immunother Cancer; Tryptophan potentiates CD8+ T cells against cancer cells by TRIP12 tryptophanylation and surface PD-1 downregulation.; Authors - Rui Qin
human	WB	Theranostics; Enhanced cancer therapy through synergetic photodynamic/immune checkpoint blockade mediated by a liposomal conjugate comprised of porphyrin and IDO inhibitor.; Authors - Zeqian Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (01-28-2022)

Worked well with a 1 hour incubation at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: A549

IDO1 Antibody Western Blot validation (1:2000 dilution) in A549 (Cat no:13268-1-AP)

FH

Hala (Verified Customer) (09-18-2020)

I am very satisfied of this antibody and of the interaction with your company

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: macrophages and hela cells

IDO1 Antibody Western Blot validation (1:1000 dilution) in macrophages and hela cells (Cat no:13268-1-AP)

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