Anticorps Polyclonal de lapin anti-IFI30

• Phare
• Validé par KD/KO

IFI30 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 11597-1-AP

Synonymes

IP30, IFI 30, GILT, Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase

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Galerie de données de validation

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  WB analysis of COLO 320 using 11597-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11597-1-AP (IFI30 Antibody) at dilution of 1:6000 incubated at 4 degree celsius over night.

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  IP experiment of COLO 320 using 11597-1-AP

  IP result of anti-IFI30 (IP:11597-1-AP, 4ug; Detection:11597-1-AP 1:3000) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11597-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11597-1-AP (IFI30 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320,
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

11597-1-AP cible IFI30 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFI30 Protéine recombinante Ag2178
• Nom complet: interferon, gamma-inducible protein 30
• Masse moléculaire calculée: 261 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC021136
• Symbole du gène: IFI30
• Identification du gène (NCBI): 10437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).

Publications

Species	Application	Title
human	WB,IHC	Int J Med Sci; IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy.; Authors - Yan Fan ; KD Validated
human	WB	Phytomedicine; Nobiletin inhibits de novo FA synthesis to alleviate gastric cancer progression by regulating endoplasmic reticulum stress; Authors - Menglin Chen
human	WB	Aging (Albany NY); Identification and validation of a novel signature based on macrophage marker genes for predicting prognosis and drug response in kidney renal clear cell carcinoma by integrated analysis of single cell and bulk RNA sequencing; Authors - Xiaoxu Chen
human	IHC	Recent Pat Anticancer Drug Discov; The Prognostic Role of Interferon Gamma-inducible Protein 30 in Clear Cell Renal Cell Carcinoma with Immune Infiltrates; Authors - Wu Xu
		Am J Physiol Cell Physiol; CFTR and PC2, partners in the primary cilia in autosomal dominant polycystic kidney disease; Authors - Murali K Yanda
human,mouse	WB,IHC,IF	Cancer Innov; Investigating the Mechanism of IFN-γ-Inducible Lysosomal Thiol Reductase-Mediated Inhibition of Breast Cancer Cell Proliferation; Authors - Qin Liu ; KD Validated

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