Anticorps Polyclonal de lapin anti-IFIH1/MDA5

• Phare
• Validé par KD/KO

IFIH1/MDA5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

42

N° de cat : 21775-1-AP

Synonymes

Helicard, Helicase with 2 CARD domains, Hlcd, IDDM19, IFIH1, IFIH1/MDA5, MDA 5, MDA5, RH116, RLR-2

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Galerie de données de validation

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  WB analysis of Jurkat using 21775-1-AP

  WB result of IFIH1 antibody (21775-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFIH1 transfected Jurkat cells.

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  WB analysis using 21775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1/MDA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21775-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1/MDA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21775-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21775-1-AP (IFIH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21775-1-AP

  IP result of anti-IFIH1 (IP:21775-1-AP, 4ug; Detection:21775-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 21775-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21775-1-AP (IFIH1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 21775-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using IFIH1/MDA5 antibody (21775-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab.

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  IF Staining of mouse skeletal muscle using 21775-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using IFIH1/MDA5 antibody (21775-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu de muscle squelettique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 38 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 4 publications below
• RIP: See 2 publications below

Informations sur le produit

21775-1-AP cible IFIH1/MDA5 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFIH1/MDA5 Protéine recombinante Ag16467
• Nom complet: interferon induced with helicase C domain 1
• Masse moléculaire calculée: 1025 aa, 117 kDa
• Poids moléculaire observé: 117-125 kDa
• Numéro d’acquisition GenBank: BC111750
• Symbole du gène: IFIH1
• Identification du gène (NCBI): 64135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
	WB,RIP	Cell Stem Cell; ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress.; Authors - Diana Guallar
human	WB	Nat Commun; MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.; Authors - Peipei Zhou
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio
human	WB	Nat Commun; HIV-1 intron-containing RNA expression induces innate immune activation and T cell dysfunction.; Authors - Hisashi Akiyama
human	WB	EMBO J; CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS; Authors - Jia Yi ; KD Validated