Anticorps Polyclonal de lapin anti-IFIT1

• Phare
• Validé par KD/KO

IFIT1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 23247-1-AP

Synonymes

G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561

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Galerie de données de validation

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  WB analysis using 23247-1-AP

  untreated and IFN beta treated HeLa cells were subjected to SDS PAGE followed by western blot with 23247-1-AP (IFIT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23247-1-AP (IFIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23247-1-AP (IFIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23247-1-AP (IFIT1 Antibody) at dilution of 1:50.

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  IHC staining of human liver cancer using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23247-1-AP (IFIT1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23247-1-AP (IFIT1 Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 23247-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23247-1-AP (IFIT1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 23247-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IFIT1 antibody (23247-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: IFN beta treated HeLa cells,
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

23247-1-AP cible IFIT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFIT1 Protéine recombinante Ag19691
• Nom complet: interferon-induced protein with tetratricopeptide repeats 1
• Masse moléculaire calculée: 478 aa, 55 kDa
• Poids moléculaire observé: 55 KDa
• Numéro d’acquisition GenBank: BC007091
• Symbole du gène: IFIT1
• Identification du gène (NCBI): 3434
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	WB	Int J Mol Sci; Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production; Authors - Sayuri Sakuragi
human	WB	J Neuroinflammation; Progression of pathology in PINK1-deficient mouse brain from splicing via ubiquitination, ER stress, and mitophagy changes to neuroinflammation.; Authors - Sylvia Torres-Odio
human	WB,IHC	Cell Oncol (Dordr); IFIT1 modulates the proliferation, migration and invasion of pancreatic cancer cells via Wnt/β-catenin signaling.; Authors - Tian-Hao Li ; KD Validated
human	WB,IHC	Front Oncol; COL8A1 Promotes NSCLC Progression Through IFIT1/IFIT3-Mediated EGFR Activation.; Authors - Xiangyi Zan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christin (Verified Customer) (03-28-2023)

Very nice antibody for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human iPSC-derived macrophages and microglia