Anticorps Polyclonal de lapin anti-IFITM1

• Phare
• Validé par KD/KO

IFITM1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

26

N° de cat : 11727-3-AP

Synonymes

9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 11727-3-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11727-3-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11727-3-AP (IFITM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of ROS1728 using 11727-3-AP

  IP Result of anti-IFITM1 (IP:11727-3-AP, 4ug; Detection:11727-3-AP 1:500) with ROS1728 cells lysate 3200ug.

• 

  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11727-3-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11727-3-AP (IFITM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of K-562 using 11727-3-AP

  1X10^6 K-562 cells were stained with 0.2ug IFITM1 antibody (11727-3-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules ROS1728
• Résultats positifs en IHC: tissu de lymphome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 4 publications below

Informations sur le produit

11727-3-AP cible IFITM1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, Singe vert d'Afrique
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFITM1 Protéine recombinante Ag2320
• Nom complet: interferon induced transmembrane protein 1 (9-27)
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC000897
• Symbole du gène: IFITM1
• Identification du gène (NCBI): 8519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.

Publications

Species	Application	Title
human	WB	Nature; Mitotic progression following DNA damage enables pattern recognition within micronuclei.; Authors - Shane M Harding
human	FC	Nature; Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.; Authors - Edward J Grow ; KD Validated
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Mol Ther; Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus.; Authors - Sarah Aref
human	WB	Nat Commun; IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses; Authors - M. Clement
human	WB	Cell Rep; IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.; Authors - Jingyou Yu ; KD Validated

Produits associés

IFITM3 Polyclonal antibody

11714-1-AP

IFITM2 Polyclonal antibody

12769-1-AP

IFIT3 Polyclonal antibody

15201-1-AP

IFIT1 Polyclonal antibody

23247-1-AP

Caveolin-1 Polyclonal antibody

16447-1-AP

ISG15 Polyclonal antibody

15981-1-AP

Beta Catenin Polyclonal antibody

51067-2-AP