Anticorps Polyclonal de lapin anti-IFITM2

• Phare
• Validé par KD/KO

IFITM2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

39

N° de cat : 12769-1-AP

Synonymes

1 8D, IFITM2

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Galerie de données de validation

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  WB analysis using 12769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12769-1-AP (IFITM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12769-1-AP

  WB result of IFITM2 antibody (12769-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFITM2 transfected HepG2 cells.

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  IP experiment of HepG2 using 12769-1-AP

  IP result of anti-IFITM2(IP:12769-1-AP, 4ug; Detection:12769-1-AP 1:10000) with HepG2 cells lysate 1360 ug.

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  IHC staining of human breast cancer using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12769-1-AP (IFITM2 Antibody) at dilution of 1:50.

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  IHC staining of mouse brain using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12769-1-AP (IFITM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12769-1-AP (IFITM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12769-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 12769-1-AP (IFITM2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 12769-1-AP

  Immunofluorescent analysis of HepG2 cells using 12769-1-AP (IFITM2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of MCF-7 using 12769-1-AP

  1X10^6 MCF-7 cells were stained with 0.2 ug Anti-Human IFITM2 (12769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 32 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• FC: See 4 publications below

Informations sur le produit

12769-1-AP cible IFITM2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFITM2 Protéine recombinante Ag3451
• Nom complet: interferon induced transmembrane protein 2 (1-8D)
• Masse moléculaire calculée: 132 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC009696
• Symbole du gène: IFITM2
• Identification du gène (NCBI): 10581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.

Publications

Species	Application	Title
human	WB	Cell; The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.; Authors - Abraham L Brass
human	WB	Cell Stem Cell; PMID: 30416070; Authors - Carolina Petrillo
human	WB	Cell Host Microbe; Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.; Authors - Toshana L Foster ; KD Validated
human	WB, FC	Cell Host Microbe; IFITM Proteins Incorporated into HIV-1 Virions Impair Viral Fusion and Spread.; Authors - Alex A Compton ; KD Validated
human	WB	Nat Commun; IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses; Authors - M. Clement
mouse	WB	Nat Commun; BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages.; Authors - Alessandra Rosati ; KD Validated

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