Anticorps Recombinant de lapin anti-IFN-beta

IFN-beta Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240230B6

N° de cat : 98082-1-RR

Synonymes

Ifnb1, 240230B6, Ifb, IFN beta, IFNB

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98082-1-RR

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR, Clone:240230B6) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were stained with CoraLite® Plus 647 Anti-Mouse CD19 before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mouse splenocytes using 98082-1-RR

  1x10^6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IFN-beta Rabbit Recombinant Antibody (98082-1-RR, Clone:240230B6)(red) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98082-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98082-1-RR against Mouse IFN-beta were performed. The affinity constant is 1.91 nM.

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Applications testées

• Résultats positifs en FC (Intra): splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98082-1-RR cible IFN-beta dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFN-beta Protéine recombinante Eg0660
• Nom complet: interferon beta 1, fibroblast
• Masse moléculaire calculée: 22KD
• Numéro d’acquisition GenBank: NM_010510.1
• Symbole du gène: Ifnb1
• Identification du gène (NCBI): 15977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Interferon-beta (IFN-β) is a type I interferon, which is a group of signaling proteins made and released by host cells in response to the presence of several viruses, including double-stranded RNA produced by many viruses. IFN-β is a single protein species and is molecularly related to IFN-α subtypes, although it is antigenically distinct from them. It is mainly produced by fibroblasts and plays a crucial role in the innate immune response against viruses. IFN-β can inhibit viral replication by interfering with the virus's RNA or DNA, thus suppressing viral growth. It also enhances the cytotoxic activity of natural killer (NK) cells and promotes the expression of major histocompatibility complex (MHC) class I molecules.

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