Anticorps Monoclonal anti-IFN gamma

IFN gamma Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

XMG1.2

N° de cat : CL488-65153

Synonymes

Ifg, IFN g, IFN gamma, IFN γ, Ifng, IFNγ, IFN-γ, interferon gamma



Applications testées

Résultats positifs en cytométriesplénocytes de souris

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65153 cible IFN gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Rat / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet interferon gamma
Masse moléculaire calculée17 kDa
Numéro d’acquisition GenBankBC119063
Symbole du gène Ifng
Identification du gène (NCBI) 15978
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 IFN gamma antibody CL488-65153Download protocol
Standard Protocols
Click here to view our Standard Protocols