Anticorps Recombinant de lapin anti-IFN-gamma

IFN-gamma Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6K20

N° de cat : CL488-98001

Synonymes

IFN γ, Ifng, IFNG2, interferon gamma

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Galerie de données de validation

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  FC experiment of rat splenocytes using CL488-98001

  1x10^6 unstimulated or ConA+PMA and ionomycin stimulated (in the presence of protein transport inhibitors) rat splenocytes cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Rat IFN-gamma (6K20) Rabbit Recombinant Antibody (CL488-98001, Clone:6K20), and 0.25 ug APC Anti-Rat CD4 (OX-35) (APC-65179, Clone: OX-35), and 0.25 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): ConA, PMA and ionomycin stimulated rat splenocytes
• Résultats positifs en cytométrie: ConA, PMA and ionomycin stimulated rat splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-98001 cible IFN-gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interferon gamma
• Masse moléculaire calculée: 18 kDa
• Numéro d’acquisition GenBank: NM_138880
• Symbole du gène: IFN-gamma
• Identification du gène (NCBI): 25712
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interferon-gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

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