Anticorps Monoclonal anti-IFN-gamma

IFN-gamma Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG1, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

XMG1.2

N° de cat : CL647-65153

Synonymes

IFN gamma, Ifng, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN g

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Galerie de données de validation

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  FC experiment of C57BL/6 Th1-polarized splenocytes using CL647-65153

  1x10^6 PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Mouse IFN gamma (CL647-65153, Clone:XMG1.2), and 0.13 ug CoraLite® Plus 647 Rat IgG1 Isotype Control (HRPN) (CL647-65212, Clone: HRPN), and 0.13 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA and ionomycin Stimulate C57BL/6 Th1-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

CL647-65153 cible IFN-gamma dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Purified Recombinant Mouse IFN gamma
• Nom complet: interferon gamma
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: BC119063
• Symbole du gène: IFN gamma
• Identification du gène (NCBI): 15978
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

Publications

Species	Application	Title
	FC	Int J Mol Sci; Development of a Vaccine Candidate Based on Surface-Displayed Particles of Mycobacterium tuberculosis from the MTB39A Protein; Authors - Pu Wang

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