Anticorps Polyclonal de lapin anti-IFNGR1

• Phare
• Validé par KD/KO

IFNGR1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10808-1-AP

Synonymes

CD119, CDw119, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 10808-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10808-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10808-1-AP (IFNGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10808-1-AP

  IP Result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:500) with HepG2 cells lysate 1800ug.

• 

  IP experiment of HepG2 using 10808-1-AP

  IP Result of anti-IFNGR1 (IP:10808-1-AP, 4ug; Detection:10808-1-AP 1:300) with HepG2 cells lysate 3000ug.

• 

  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10808-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10808-1-AP (IFNGR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 10808-1-AP

  1X10^6 HepG2 cells were stained with .2ug IFNGR1 antibody (10808-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10808-1-AP cible IFNGR1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFNGR1 Protéine recombinante Ag1114
• Nom complet: interferon gamma receptor 1
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC005333
• Symbole du gène: IFNGR1
• Identification du gène (NCBI): 3459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Publications

Species	Application	Title
mouse	IHC	Cancer Discov; Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.; Authors - Wan Du
mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Dev Cell; Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells.; Authors - Elena Parmigiani
human,mouse	WB	Oncogene; Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia; Authors - Xiaoling Xie ; KD Validated
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ
mouse	WB	J Biol Chem; PMID: 28174303; Authors - Barbara Kroczynska

Produits associés

IFN Gamma Polyclonal antibody

15365-1-AP

IFNGR2 Polyclonal antibody

10266-1-AP

STAT1 Polyclonal antibody

10144-2-AP

JAK2 Polyclonal antibody

17670-1-AP

JAK1 Monoclonal antibody

66466-1-Ig