Anticorps Polyclonal de lapin anti-IFN-gamma R2

IFN-gamma R2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 10266-1-AP

Synonymes

IFNGR2, AF 1, AF-1, IFGR2, IFN gamma R2

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Galerie de données de validation

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  WB analysis using 10266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10266-1-AP

  IP result of anti-IFNGR2 (IP:10266-1-AP, 4ug; Detection:10266-1-AP 1:500) with A549 cells lysate 1800ug.

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  IHC staining of human breast cancer using 10266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IF: See 3 publications below

Informations sur le produit

10266-1-AP cible IFN-gamma R2 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFN-gamma R2 Protéine recombinante Ag0317
• Nom complet: interferon gamma receptor 2 (interferon gamma transducer 1)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC003624
• Symbole du gène: IFNGR2
• Identification du gène (NCBI): 3460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit which attaches to IFN-γ with a significantly lower affinity. Two subunits bind one IFN-γ dimer. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Publications

Species	Application	Title
mouse	WB	Science; Aberrant type 1 immunity drives susceptibility to mucosal fungal infections.; Authors - Timothy J Break
mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Cancer Commun (Lond); MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.; Authors - Qingnan Wu
human	WB	PLoS One; Impact of adipose tissue or umbilical cord derived mesenchymal stem cells on the immunogenicity of human cord blood derived endothelial progenitor cells.; Authors - Kefang Tan
human	WB	Biomol Biomed; Identification of RUNX1 and IFNGR2 as prognostic-related biomarkers correlated with immune infiltration and subtype differentiation of low-grade glioma; Authors -
human	WB,IF	Sci Adv; Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling; Authors - Benjamin Ledoux

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