Anticorps Polyclonal de lapin anti-IFNGR2

IFNGR2 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10266-1-AP

Synonymes

AF 1, IFGR2, IFN gamma R2, IFN gamma receptor 2, IFNGR2, IFNGT1, Interferon gamma receptor 2, Interferon gamma transducer 1

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Galerie de données de validation

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  WB analysis of A549 using 10266-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10266-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10266-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10266-1-AP (IFNGR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10266-1-AP

  IP Result of anti-IFNGR2 (IP:10266-1-AP, 4ug; Detection:10266-1-AP 1:500) with A549 cells lysate 1800ug.

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  IHC staining of human breast cancer using 10266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10266-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10266-1-AP (IFNGR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 10266-1-AP

  1X10^6 HeLa cells were stained with 0.2ug IFNGR2 antibody (10266-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

10266-1-AP cible IFNGR2 dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFNGR2 Protéine recombinante Ag0317
• Nom complet: interferon gamma receptor 2 (interferon gamma transducer 1)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC003624
• Symbole du gène: IFNGR2
• Identification du gène (NCBI): 3460
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit which attaches to IFN-γ with a significantly lower affinity. Two subunits bind one IFN-γ dimer. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Publications

Species	Application	Title
mouse	WB	Science; Aberrant type 1 immunity drives susceptibility to mucosal fungal infections.; Authors - Timothy J Break
mouse	WB	Nat Commun; DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.; Authors - Baiwen Chen
human	WB	Cancer Commun (Lond); MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.; Authors - Qingnan Wu
human	WB	PLoS One; Impact of adipose tissue or umbilical cord derived mesenchymal stem cells on the immunogenicity of human cord blood derived endothelial progenitor cells.; Authors - Kefang Tan
human	WB	Biomol Biomed; Identification of RUNX1 and IFNGR2 as prognostic-related biomarkers correlated with immune infiltration and subtype differentiation of low-grade glioma; Authors -

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