Anticorps Polyclonal de lapin anti-IFT20

• Phare
• Validé par KD/KO

IFT20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(82)

Avis (3)

N° de cat : 13615-1-AP

Synonymes

Intraflagellar transport protein 20 homolog, hIFT20

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Galerie de données de validation

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  WB analysis of HEK-293 using 13615-1-AP

  WB result of IFT20 antibody (13615-1-AP, 1:500) with si-Control and si-IFT20 transfected HEK293 cells.

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  WB analysis using 13615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 13615-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13615-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13615-1-AP

  IP result of anti-IFT20 (IP:13615-1-AP, 3ug; Detection:13615-1-AP 1:700) with mouse testis tissue lysate 4000ug.

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  IHC staining of human endometrial cancer using 13615-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human endometrial cancer using 13615-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 13615-1-AP

  IF result (localization to Golgi-like structures) of anti-IFT20 (13615-1-AP, 1:50) with serum-starved hTERT-RPE1 (MoTH fixed) by Dr. Moshe Kim.

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  IF Staining of MDCK using 13615-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 13615-1-AP (IFT20 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MDCK, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules hTERT-RPE1, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:800
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 16 publications below
• WB: See 47 publications below
• IHC: See 6 publications below
• IF: See 49 publications below
• IP: See 2 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13615-1-AP cible IFT20 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, canin, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT20 Protéine recombinante Ag4521
• Nom complet: intraflagellar transport 20 homolog (Chlamydomonas)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 15-18 kDa
• Numéro d’acquisition GenBank: BC038094
• Symbole du gène: IFT20
• Identification du gène (NCBI): 90410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

Publications

Species	Application	Title
mouse	WB	Nature; Functional interaction between autophagy and ciliogenesis.; Authors - Pampliega Olatz O
mouse	IF	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
human	IF	Nat Cell Biol; Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.; Authors - Quanlong Lu
human	WB	Sci Adv; An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.; Authors - Molly M Wilson
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	WB	Proc Natl Acad Sci U S A; EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.; Authors - Vincent J Guen ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-18-2024)

I did the standard iF protocol on the classic LIGHT2 cells used to study Hh signalling that depends on the primary cilia: PFA 4% 10 min cold methanol 5 min 1h in 1% BSA in PBST 1/150 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS I'm not convinced as the background is super dotty and quite important. Maybe the conditions must be optimized but it doesn't look promising

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/150
• Cell Tissue Type: LIGHT2

IFT20 Antibody Immunofluorescence validation (1/150 dilution) in LIGHT2 (Cat no:13615-1-AP)

FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and IF for cilia (1:200) and got good results.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

FH

Boyan (Verified Customer) (03-15-2019)

Excellent for IF labelling of the Golgi under PFA fixation.

• Applications: Immunofluorescence,
• Cell Tissue Type: MEF

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